Lambda-Max Scans

The same trial mixture as before which separated into three peaks under isocratic conditions, separated into four peaks under binary gradient conditions.

Each peak was scanned at its maximum - Lambda max scan – and the spectra displayed whilst the chromatogram was simultaneously displayed and quantified by integration to provide baseline corrected peak areas and peak heights.

Peaks 1, 2 and 4 are quantitatively similar and all have peak maxima close to 222 and 273nm.

Peak 3 scan is clearly very different having maxima near 234, 268, 316, and 330nm.

Optimum monitoring wavelengths for each peak may be chosen from these scans.