Integration

This chromatogram shows the separation of a four component mixture that has been integrated using the integration parameters shown in the bottom window. The components are defined by retention time but not yet identified and named in the peak table, the peak starts and ends are also defined and tabulated. The integrated peak areas and peak heights are calculated and included in the peak table but the chromatogram has not yet been quantified by reference to a calibration curve. The dialogue box provides a means of quickly re-scaling the axes of the chromatogram if required.

Selecting a Component

Integration parameters are rapidly accessed and changed, when necessary, using a dialogue box that allows individual parameters in the integration process to be modified. By selecting a component in the peak table it is instantly identified in the graphical display, using a colour infill, so that peak definition by start and end points, using the current integration parameters can be viewed and quickly optimised. The four major components of the chromatogram are identified and named in the component table and peak number 3 (pyrene) has been quantified by reference to a prior calibration curve.

Calibration

PowerStream will automatically define a time window in which a peak should appear, to be correctly recognised and named in the component peak table. The width of the time window is set after measuring the widest half widths of the asymmetrical peaks, i.e. on the widest trailing side of the peak. The default value can be changed by the user.

When a peak is highlighted in the peak component table, the time window is displayed superimposed on the appropriate peak. If the peak has been calibrated the concentration, peak height and area tables are displayed together with the associated calibration curve as shown in the lower window. In this case a linear curve fit was chosen. A choice of linear, quadratic or cubic fit is provided.

Report Formatting

The final report may be user configured and a template defined to include as much or as little information as is required. The basic format will have four segments to a single page, the header information, the chromatogram, the results table and a footer. Each of these separate sections can be sized and their contents defined, easily and rapidly using dialogue boxes.

Should a larger chromatogram format (up to a whole page), or all and every detail of the results, be required, a second page may be additionally defined.

Calibration

• Linear
• Quadratic
• Cubic

Overlays

• With Offset
• Subtraction
• Addition
• Manipulation

Integration

• Automatic Baseline
• Manual Baseline
• Drop Line
• Sloping Baseline
• Tangential Skimming
• Exponential Skimming
• Selectable Peak Detection
• Sensitivity

Help Screens

• Complete Operating Guides