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Dedicated to Molecular Biology
The GeneQuest Analyser is a spectrophotometer dedicated to the
requirements of the Molecular Biology Laboratory. Fully programmed
methods include those for RNA, ds DNA, ss DNA, oligonucleotide primer,
proteins, bacterial growth and cell culture measurements and molecular
calculations, including theoretical thermal melt.
Ultra Micro-Samples
Measurements are possible for a wide range of sample volumes, from
2000µl to 5µl using an appropriately selected cell in
the ultra microcell holder fitted as standard in the GeneQuest.
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Standard Software
A260/A280 Ratio
A260/A280 with background correction 320nm
Direct UV method for protein at 280nm
A230/A260 Ratio |
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A 230/A260 with background correction 320nm
ss DNA concentration
ds DNA concentration
RNA concentration |
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Sample Cell Selection
A range of UV transmitting silica cells is available. The listing
given here enables the cell appropriate to the sample volume to
be chosen. All cells except the standard must be of the self masking
type. Using a 10mm pathlength cell an absorbance value of 1 is estimated
for a concentration of 50µl/ml of ds DNA.
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Volume
(uL) |
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Pathlength
(mm) |
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Cell Description |
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Cell Type |
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Part Number |
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| 2000 |
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10 |
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Standard |
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A |
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202 07 26 |
| 400 |
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10 |
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Semi-micro, thick base |
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B |
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202 07 57 |
| 200 |
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10 |
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Micro, thick base |
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C |
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202 07 58 |
| 70 |
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10 |
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Ultra micro |
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D |
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202 07 15 |
| 50 |
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10 |
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Ultra micro |
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E |
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202 07 01 |
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use holder 2020 29 00 |
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| 10 |
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10 |
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Ultra micro, pipette filling |
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F |
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202 07 64 |
| 1000 |
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5 |
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Standard – use spacer |
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A |
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202 07 25 |
| 5 |
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5 |
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Ultra micro, pipette filling |
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F |
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202 07 63 |
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Simplicity of Operation
Insert the reference, press ZERO, then insert the sample and press
RUN to obtain a fully documented report complete with header, sample
number and space for the operators name etc. Operation could not
be more simple. Operators may create and store up to 30 of their
own methods with the method storage option.
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Automatic Wavelength Selection
All the fully programmed methods are selected from a menu and displayed
with headers, prompts and details on the large display screen on
which information may be scrolled for viewing.
All wavelengths for any given assay are automatically selected
and measured. Results are displayed on the screen and a printout,
timed and dated, is available on an external printer, or an internal
printer in the case of the CE2302.
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Enhanced Performance
The excellent accuracy of the GeneQuest for normal samples is further
enhanced for poorly transmitting samples by an automatic integration
process initiated in response to a flashing alert message.
Validation
To fulfil legislative requirements and GLP, onboard software allows
for performance validation using filter sets calibrated by Cecil
Instruments and supplied with calibration certificates traceable
to NPL standards. Methods for liquid samples are also provided.
Benefits
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Full screen display of menus, prompts and results
Software options password enabled at any time by
E-SEF
Storage for 30 user methods, password protected
Warning of poor sample transmission
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Signal enhancement for poor sample transmission
Plot of curve fitted to standards
Full UV range available to create methods
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