Bio Spectrophotometers
Specially pre-programmed for bioanalysis and molecular biology,
the BioQuest and BioAquarius also have all the normal functions
of UV visible spectrophotometers, scanning, time course plots, single
wavelength measurements in Absorbance, Concentration or Transmittance,
cell and wavelength programming, method storage etc.
Protein Analysis
Preprogrammed methods for all the normal protein analyses are provided,
these include the following methods:
Bradford Biuret
Lowry (low sens.) BCA
Lowry (high sens.) Direct UV
Assays may also be carried out using the Warburg and Christian
2 or 3 wavelength methods and the Kalb and Bernlohr procedures,
all of which are preprogrammed.
Nucleic Acid Assays
Concentration and purity may be determined by the methods of Warburg
and Christian, Kalb and Bernlohr or using user selected parameters.
DNA, ssDNA, dsDNA, RNA and oligonucleotides may be quantified and
examined for purity using two or three wavelength ratio methods.
Molecular concentration may be determined for Oligo Samples and
theoretical melting points for DNA samples calculated using the
nearest neighbour interaction model.
Concentration Curve Fitting
For more accurate measurements and for measurements where Beer's
law is not obeyed a straight line quadratic or cubic curve may be
fitted to up to 30 standards or replicate standards. Editing of
suspect standards may be carried out and the final curve stored
in code protected memory for future use.
Reaction Kinetics
Both single and up to 6 cell multi-cell Kinetics are readily carried
out. Water or thermoelectric temperature control is available also
sample stirring.
Reaction curves are displayed on screen and regression analysis
applied to the user selected part of the curves to calculate results.
Advance Kinetics is possible providing such plots as Michaelis
Menton and Lineweaver Burk etc., from which Km and Vmax are automatically
calculated.
Small Sample Measurements
A return Micro Sipette system is available which uses a 75µl
cell, measurements on samples of 300µl or less may be made
with low levels of cross contamination.
A special holder accommodates cells of 50µl for measuring
valuable small samples. These cells may be used in both sample and
reference beams in the BioAquarius instrument.
For the ultimate in small sampling a special pipette loaded 5µl
cell with 5mm pathlength is available.
Experimental Thermal Melt ™
Thermoelectric temperature programming is available for thermal
melt (Tm) determinations.
A fully annotated plot of absorbance against temperature is displayed
on screen. The data may be reprocessed, overlaid, stored or transferred
to a PC.
Method Generation and Storage
All preprogrammed methods may be modified by the user to meet their
requirements. Any modified method may then be stored as an additional
method in code protected memory.
DataStream - Data to PC
The DataStream system allows all data to be exported rapidly and
with great ease, to a PC for incorporation into a spreadsheet or
mathematical package of the users choice - Excel, Grafit etc - for
report generation and mathematical manipulation.
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