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Formulae for measurements at up to 10 wavelengths may be entered rapidly. Programming and formatting of results is automatic at entry without user intervention. Multi-
In the concentration measurement mode samples may be measured either by using a calibration standard, or entering a known factor relating concentration to Absorbance.
Calibration Curve and Line Fitting
A straight line, quadratic or cubic curve may be fitted to up to 30 standards, with or without an intercept at the origin. Standards may be deleted and replaced and a new curve fitted.
Single and multi-
Multi Wavelength Assays
Assays are provided using either two or three wavelengths. A wine colour assay is provided which may use up to ten different wavelengths. Computations at up to four wavelengths are also provided.
Protein analyses may be made using either the 2 or 3 wavelength methods of Warburg and Christian. Also programmed are techniques and analytical procedures for assays by Lowry, Bradford, Biuret and BCA methods.
Ratio And Difference
Absorbance ratioing at 280/260nm, or two user selected wavelengths, is provided for DNA purity assays. The scaled difference of Absorbance values at two wavelengths is also provided.
Using the various cell changers, measurements may be made on up to 4, 6 or 8 cells at a single wavelength with cycle time and number of cycles entered by the operator. A cell and wavelength program may be combined.
Measurements may be programmed for 10 different operator selected wavelengths with the cycle time and number of cycles selected by the operator. A wavelength and cell program may be combined.
Cells And Flowcells
As well as 10mm cells and flowcells, holders are available for rectangular and cylindrical cells up to 100mm pathlength. A holder for solid samples is also available.
Automatic Cell Changers
Automatic changers are available for 4, 6 or 8 cells, providing rapid sample changing or full programming.
Samples down to 300 microlitres may be measured in a 10mm cell. Sampling is under control of the instruments microprocessor system. Samples may be saved or pumped to waste.
Small or valuable samples down to 50 microlitres or less may be measured using a specially designed cell and holder.
A special precision cell holder is available for ultra-
Sample Temperature Control
Water and thermoelectric temperature control is available for single cells and automatic cell changers. Temperature is set from the control panel and displayed on the screen.
Temperature programming of samples using a single cell or 4 cell changer is possible using a Thermoelectric controller with a range of 10°-
Automatic batch sampling for up to 100 samples is available. The pump fits within the sample compartment and volume etc is set from the control panel and displayed on screen. Batch number entry is provided.
Versions of the instruments fitted with an 8 cell changer, special piping port and lid, are available for dissolution testing using software and a PC.
Simplicity of operation, for even complex tasks, coupled with a wide range of software and accessories, makes the Aurius and Super Aurius instruments ideal multi-
User Written Programs, using Assay Program E-
Allows the text entry of any formula with up to 76 characters. For complex calculations up to 20 such formulae may be entered and linked for formidable computing power without a PC. Assays may use up to 10 wavelengths and 8 cells in any automatic programmed method. Once entered programmes may be stored, as methods, security protected. Tabulation of results is automatic and the ease and speed of use makes Assay Program a very powerful tool.
Fast Program Any Assay
Any quantitative assay may be rapidly input for automatic computation using the Cecil Assay Program software module. No programming skill is required by the operator. Transcription and calculation errors are avoided and validation is simple as no PC is used. Calculations for a series of Carboxyhaemoglobin samples is shown below with the formulae used.
Absorbance ratios are standard and quantitative assays using both two and three wavelengths are available, including the methods of Warburg & Christian for nucleic acids and proteins.
Super Aurius Performance
The Super Aurius range offers un compromised performance and analytical power only available in much larger and more expensive instruments. With 1.8nm optical bandwidth even closely spaced or narrow bands may be resolved, as illustrated by the benzene vapour spectrum shown here.
With the added power of the 'Quant S' software module, scans may be overlayed and differencing, stripping and band area computation carried out.
Storage for 30 spectra, with full security, is provided. Derivative spectra are also standard, with multi-
Spectral information is often masked by overlapping absorption bands, sloping background absorption, or the occurrence of a small peak or shoulder on the side of a broad absorption band.
Derivative spectroscopy may be used to enhance fine detail, reduce the effects of a sloping background and accurately locate a masked band for measurement.
For accurate quantitative work it is often required to determine the area or height of an absorption band corrected for background absorption.
A very easy to use programme is provided for this purpose. The analytical result is displayed on the screen, with construction lines, enabling the quality of the chosen parameters to be assessed.
Band quantification may be stored as a method security protected.
The Super Aurius Series offers multi-
Regression analysis is applied to the user selected portion of the reaction curves (t1-
Data may be reprocessed and curves stored security protected.
Water and thermoelectric sample temperature control are available, also sample stirring.
Use of the Spectrophotometers with a PC
Cecil DataStream provides for rapid transfer of raw data, or data processed and formatted by the spectrophotometer, to a PC for instant display. Scans, single measurements, programmed measurements and sophisticated analytical procedures may all be transferred with great ease.
Transferred data may be saved on disk or incorporated into a spread sheet or maths pack. Incorporation into Microsoft Excel is shown here for display, manipulation, graphical presentation, archiving or report generation.
Simplicity of operation is a key feature as any familiar spreadsheet or maths package -
Raw Kinetics data may be transferred by DataStream into 'Grafit'. The Lineweaver Burk transformation, shown below, was automatically plotted together with the values of Km and Vmax. Other transformations may be used.
Both ranges are offered equipped for Dissolution Assay applications using an autochanger for up to 8 cells and with a piping port. Complete systems are offered, as shown here, including dissolution baths, pumps and dissolution software. The Super Aurius should be used when a narrow 1.8nm bandpass is required.
Instrument performance may be validated using a comprehensive software module available on all instruments and which may be released by E-
Tests are provided for laboratories subject to regulatory control or following good G.L.P. These include a range of Pharmacopoeia tests (EP/BP/USP), a selection of which is shown here.
The very accurate method for validating optical bandwidth uses the narrow deuterium emission line at 656.1nm. The width, at half height, of the measured band due to the emission line measures the actual bandwidth. The band is drawn and the half width automatically documented.
The Pharmacopoeia (EP/BP) test for Absorbance accuracy is one of the tests provided. The test measures the values of peaks and valleys in the Absorbance spectrum of a 60mg/L solution of Potassium Dichromate in a 10mm pathlength cuvette. The results of such a test run are shown here.
Straylight may be measured using the Pharmacopoeia (EP/BP) test shown here. This test uses a 1.2% W/V solution of Potassium Chloride in water, measured against water in the reference beam. An Absorbance in excess of 2A must be measured at a wavelength of 200nm.
The ASTMS tests at 220nm and 340nm are more widely used and are provided for.
Validation Using Filters
Absorbance and Wavelength validation are most conveniently carried out using filter sets calibrated by Cecil Instruments on its reference spectrophotometer against standards certified by the NPL. Full validation programmes for these tests are provided.
Cecil DataStream software provides fast transfer of all data to a PC for incorporation into a spread sheet or mathematical package of the users choice. Fast and easy to use.
Self Test and Calibration
An automatic test procedure, at switch-
Method Operation and Storage
Up to 30 sets of instrument parameters, including concentration curves may be stored, security code protected. When recalled, instrument parameters are automatically set, avoiding errors.
Software Enhancement By E-
Data Storage With Security
Data, including spectra, concentration curves, kinetics and time course plots may be stored in safe memory, security code protected against tamper or deletion.
Display Scrolling For Wide Viewing
Use of the display scrolling facility enhances the effective screen width to 550nm and enables spectra and data up to six screen widths to be viewed.
Scans, time course plots, and reaction curves may all be reprocessed to any required format on the display screen and then plotted on the integral or an external printer.
USB and Serial RS232c Ports
Computers may be connected via one of the two ports provided. Data may be downloaded to a computer for mass spectral storage, processed or transferred to a spread sheet.
Software and certified standards enable wavelength accuracy, Absorbance accuracy, bandwidth etc. to be validated. A timed and dated report includes the serial number of the instrument.
Scans at up to 4000nm/min are presented on screen or printed with all peaks annotated with wavelength and Absorbance. Tables are also presented listing all peaks and troughs with values. Scan is an option on the Aurius range.
Derivative spectra are readily produced, with selectable smoothing, either alone or superimposed on the fundamental, with or without an offset. Scaling is automatic, with all peaks and troughs quantified.
Up to six scans, including stored scans, may be overlayed, with or without offsets for convenient comparisons or further manipulation.
Dynamic Scan Recall
The last eight scans are automatically retained for recall by selection from a list and may be reprocessed, overlayed or transferred to protected memory for future use.
Band Area Height Computation
Band areas with correction for sloping background absorption are readily quantified, with all details and construction displayed. Band areas may also be computed for derivative spectra to facilitate difficult quantification.
Spectra may be stored in safe memory for future recall. Each spectrum is automatically allocated a recall number, when stored, and may be security code protected against tamper or deletion.
Scans, automatic, single or repeat may be made of a cell program of up to 4, 6 or 8 cells. A programmed sequence of scans may also be made of a single sample.
Time Course Plots
Changing samples or flows may be examined and displayed by making a time course plot. Data may be reprocessed or stored, security code protected, with an automatically assigned recall number.
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