Aurius and Super Aurius  Spectrophotometers

Further Information

Quantification


Assay Program

Formulae for measurements at up to 10 wavelengths may be entered rapidly. Programming and formatting of results is automatic at entry without user intervention. Multi-sample assays are automatic and may be stored as methods.


Concentration Measurement

In the concentration measurement mode samples may be measured either by using a calibration standard, or entering a known factor relating concentration to Absorbance.


Calibration Curve and Line Fitting

A straight line, quadratic or cubic curve may be fitted to up to 30 standards, with or without an intercept at the origin. Standards may be deleted and replaced and a new curve fitted.


Reaction Kinetics

Single and multi-cell kinetic measurements are possible with plots and data displayed on screen. Data may be reprocessed and curves stored, security code protected against tamper or deletion.


Multi Wavelength Assays

Assays are provided using either two or three wavelengths. A wine colour assay is provided which may use up to ten different wavelengths. Computations at up to four wavelengths are also provided.


Protein Assays

Protein analyses may be made using either the 2 or 3 wavelength methods of Warburg and Christian. Also programmed are techniques and analytical procedures for assays by Lowry, Bradford, Biuret and BCA methods.


Ratio And Difference

Absorbance ratioing at 280/260nm, or two user selected wavelengths, is provided for DNA purity assays. The scaled difference of Absorbance values at two wavelengths is also provided.


Cell Program

Using the various cell changers, measurements may be made on up to 4, 6 or 8 cells at a single wavelength with cycle time and number of cycles entered by the operator. A cell and wavelength program may be combined.


Wavelength Program

Measurements may be programmed for 10 different operator selected wavelengths with the cycle time and number of cycles selected by the operator. A wavelength and cell program may be combined.

Sampling


Cells And Flowcells

As well as 10mm cells and flowcells, holders are available for rectangular and cylindrical cells up to 100mm pathlength. A holder for solid samples is also available.


Automatic Cell Changers

Automatic changers are available for 4, 6 or 8 cells, providing rapid sample changing or full programming.


Sipette Sampling

Samples down to 300 microlitres may be measured in a 10mm cell. Sampling is under control of the instruments microprocessor system. Samples may be saved or pumped to waste.


Micro Sampling

Small or valuable samples down to 50 microlitres or less may be measured using a specially designed cell and holder.


Ultra-Micro Sampling

A special precision cell holder is available for ultra-microcells using as little as 5 or 10µl of valuable samples. Samples are loaded into the cell by pipette.  Third party nano cells may also be used.


Sample Temperature Control

Water and thermoelectric temperature control is available for single cells and automatic cell changers. Temperature is set from the control panel and displayed on the screen.


Temperature Programming

Temperature programming of samples using a single cell or 4 cell changer is possible using a Thermoelectric controller with a range of 10°-100°C producing temperature programmes at up to 1°C per minute.


Batch Sampling

Automatic batch sampling for up to 100 samples is available. The pump fits within the sample compartment and volume etc is set from the control panel and displayed on screen. Batch number entry is provided.


Dissolution Testing

Versions of the instruments fitted with an 8 cell changer, special piping port and lid, are available for dissolution testing using  software and a PC.

Assay Programs


Flexibility

Simplicity of operation, for even complex tasks, coupled with a wide range of software and accessories, makes the Aurius and Super Aurius instruments ideal multi-user instruments, and excellent for multi-discipline laboratories.


User Written Programs, using Assay Program E-SEF software

Allows the text entry of any formula with up to 76 characters. For complex calculations up to 20 such formulae may be entered and linked for formidable computing power without a PC. Assays may use up to 10 wavelengths and 8 cells in any automatic programmed method. Once entered programmes may be stored, as methods, security protected. Tabulation of results is automatic and the ease and speed of use makes Assay Program a very powerful tool.


Fast Program Any Assay

Any quantitative assay may be rapidly input for automatic computation using the Cecil Assay Program software module. No programming skill is required by the operator.  Transcription and calculation errors are avoided and validation is simple as no PC is used. Calculations for a series of Carboxyhaemoglobin samples is shown below with the formulae used.


Multi-Wavelength  Assays

Absorbance ratios are standard and quantitative assays using both two and three wavelengths are available, including the methods of Warburg & Christian for nucleic acids and proteins.

Performance


Super Aurius Performance

The Super Aurius range offers un compromised performance and analytical power only available in much larger and more expensive instruments. With 1.8nm optical bandwidth even closely spaced or narrow bands may be resolved, as illustrated by the benzene vapour spectrum shown here.


With the added power of the 'Quant S' software module, scans may be overlayed and differencing, stripping and band area computation carried out.


Storage for 30 spectra, with full security, is provided. Derivative spectra are also standard, with multi-cell Kinetics provided by the 'Kinetics M' module.


Derivative Spectra

Spectral information is often masked by overlapping absorption bands, sloping background absorption, or the occurrence of a small peak or shoulder on the side of a broad absorption band.

Derivative spectroscopy may be used to enhance fine detail, reduce the effects of a sloping background and accurately locate a masked band for measurement.


Band Quantification

For accurate quantitative work it is often required to determine the area or height of an absorption band corrected for background absorption.


A very easy to use programme is provided for this purpose. The analytical result is displayed on the screen, with construction lines, enabling the quality of the chosen parameters to be assessed.


Band quantification may be stored as a method security protected.


Multi-Cell Kinetics

The Super Aurius Series offers multi-cell Kinetics as well as single cell Kinetics as available on the Aurius Series. Plots are displayed on the screen and printed.


Regression analysis is applied to the user selected portion of the reaction curves (t1-t2) and results are reported in Units/Litre.

Data may be reprocessed and curves stored security protected.


Water and thermoelectric sample temperature control are available, also sample stirring.

Use of the Spectrophotometers with a PC


DataStream

Cecil DataStream provides for rapid transfer of raw data, or data processed and formatted by the spectrophotometer, to a PC for instant display. Scans, single measurements, programmed measurements and sophisticated analytical procedures may all be transferred with great ease.


Transferred data may be saved on disk or incorporated into a spread sheet or maths pack. Incorporation into Microsoft Excel is shown here for display, manipulation, graphical presentation, archiving or report generation.

Simplicity of operation is a key feature as any familiar spreadsheet or maths package -Excel, Lotus 123, Grafit etc - may be used, avoiding a learning cycle and saving valuable time. Reports are printed using any printer supported by Microsoft Windows.


Advanced Kinetics

Raw Kinetics data may be transferred by DataStream into 'Grafit'. The Lineweaver Burk transformation, shown below, was automatically plotted together with the values of Km and Vmax. Other transformations may be used.


Dissolution Assay

Both ranges are offered equipped for Dissolution Assay applications using an autochanger for up to 8 cells and with a piping port. Complete systems are offered, as shown here, including dissolution baths, pumps and dissolution software. The Super Aurius should be used when a narrow 1.8nm bandpass is required.

Performance Validation


Instrument performance may be validated using a comprehensive software module available on all instruments and which may be released by E-SEF.


Tests are provided for laboratories subject to regulatory control or following good G.L.P. These include a range of Pharmacopoeia tests (EP/BP/USP), a selection of which is shown here.


Optical Bandwidth

The very accurate method for validating optical bandwidth uses the narrow deuterium emission line at 656.1nm. The width, at half height, of the measured band due to the emission line measures the actual bandwidth. The band is drawn and the half width automatically documented.


Absorbance Validation

The Pharmacopoeia (EP/BP) test for Absorbance accuracy is one of the tests provided. The test measures the values of peaks and valleys in the Absorbance spectrum of a 60mg/L solution of Potassium Dichromate in a 10mm pathlength cuvette. The results of such a test run are shown here.


Straylight

Straylight may be measured using the Pharmacopoeia (EP/BP) test shown here. This test uses a 1.2% W/V solution of Potassium Chloride in water, measured against water in the reference beam. An Absorbance in excess of 2A must be measured at a wavelength of 200nm.


The ASTMS tests at 220nm and 340nm are more widely used and are provided for.


Validation Using Filters

Absorbance and Wavelength validation are most conveniently carried out using filter sets calibrated by Cecil Instruments on its reference spectrophotometer against standards certified by the NPL. Full validation programmes for these tests are provided.


System Functions


DataStream - Fast Data Transfer

Cecil DataStream software provides fast transfer of all data to a PC for incorporation into a spread sheet or mathematical package of the users choice. Fast and easy to use.


Self Test and Calibration

An automatic test procedure, at switch-on, checks all instrument functions and calibrates wavelength against a deuterium lamp emission line. Performance is confirmed by a timed and dated report including instrument serial number.


Method Operation and Storage

Up to 30 sets of instrument parameters, including concentration curves may be stored, security code protected. When recalled, instrument parameters are automatically set, avoiding errors.


Software Enhancement By E-SEF

E-SEF - Encoded Software Enhancement Facility - allows software modules to be enabled using encoded numbers provided by telephone, fax or E-mail. Software requirements may be tailored, at any time, as required.


Data Storage With Security

Data, including spectra, concentration curves, kinetics and time course plots may be stored in safe memory, security code protected against tamper or deletion.


Display Scrolling For Wide Viewing

Use of the display scrolling facility enhances the effective screen width to 550nm and enables spectra and data up to six screen widths to be viewed.


Reprocessing

Scans, time course plots, and reaction curves may all be reprocessed to any required format on the display screen and then plotted on the integral or an external printer.


USB and Serial RS232c Ports

Computers may be connected via one of the two ports provided. Data may be downloaded to a computer for mass spectral storage, processed or transferred to a spread sheet.


Performance Validation

Software and certified standards enable wavelength accuracy, Absorbance accuracy, bandwidth etc. to be validated. A timed and dated report includes the serial number of the instrument.

Scanning


Wavelength Scan

Scans at up to 4000nm/min are presented on screen or printed with all peaks annotated with wavelength and Absorbance. Tables are also presented listing all peaks and troughs with values. Scan is an option on the Aurius range.


Derivative Spectra

Derivative spectra are readily produced, with selectable smoothing, either alone or superimposed on the fundamental, with or without an offset. Scaling is automatic, with all peaks and troughs quantified.


Overlayed Spectra

Up to six scans, including stored scans, may be overlayed, with or without offsets for convenient comparisons or further manipulation.


Dynamic Scan Recall

The last eight scans are automatically retained for recall by selection from a list and may be reprocessed, overlayed or transferred to protected memory for future use.


Band Area Height Computation

Band areas with correction for sloping background absorption are readily quantified, with all details and construction displayed. Band areas may also be computed for derivative spectra to facilitate difficult quantification.


Scan Storage

Spectra may be stored in safe memory for future recall. Each spectrum is automatically allocated a recall number, when stored, and may be security code protected against tamper or deletion.


Programmed Scans

Scans, automatic, single or repeat may be made of a cell program of up to 4, 6 or 8 cells. A programmed sequence of scans may also be made of a single sample.


Time Course Plots

Changing samples or flows may be examined and displayed by making a time course plot. Data may be reprocessed or stored, security code protected, with an automatically assigned recall number.