Reaction Kinetics
Both single cell and multi cell Kinetics are available with reaction
plots to screen and printer. Regression analysis is applied to the
user-selected portion of reaction curves and results are reported
in Units/Litre. Data may be reprocessed as required and curves stored.
Sample temperature control is available for single cells and four
and six cell changers.
Advanced Kinetics
An example of advanced kinetics is shown below where time based
reaction data generated by the spectrophotometer, has been transferred
into Grafit, fitted to a curve and transformed into a more suitable
format for mathematical interpolation and calculation. In this particular
example, the basic data plot can yield the limiting rate (Vmax),
the Michaelis constant (Km) and the initial linear portion (Vmax/Km).
This has been transformed into the Eadie-Hofstee format and plotted
to give better precision in the calculation of these important factors
and constants.
Other transformations are available such as Lineweaver-Burk and
the Hill plot for allosteric enzyme systems. |
