Micro - Sipette Sampling

Small samples or multiple samples are conveniently measured using the micro-sipette system.

Samples as small as 300µl may be measured in a 10mm pathlength cell with very low cross contamination.

All measurements are made in the same cell, enhancing accuracy.

The pump, which is mounted in the sample compartment, operates under the control of the instrument's micro-processor system.

Ultra-Micro Sampling

Valuable samples, such as biological samples prepared with costly reagents, may be measured using a special ultra-microcell and holder. With the system, samples of 50µl or even less may be accurately measured.

Reaction Kinetics

Both single cell and multi cell Kinetics are available with reaction plots to screen and printer. Regression analysis is applied to the user selected portion of reaction curves and results are reported in Units/Litre. Data may be reprocessed as required and curves stored. Sample temperature control is available for single cells and four and six cell changers.

Advanced Kinetics

An example of advanced kinetics is shown below where time based reaction data generated by the spectrophotometer, has been transferred into Grafit, fitted to a curve and transformed into a more suitable format for mathematical interpolation and calculation. In this particular example, the basic data plot can yield the limiting rate (Vmax), the Michaelis constant (Km) and the initial linear portion (Vmax/Km).

This has been transformed into the Eadie-Hofstee format and plotted to give better precision in the calculation of these important factors and constants.

Other transformations are available such as Lineweaver-Burk and the Hill plot for allosteric enzyme systems.

PC Data Manipulation

Data may be transferred to a PC for use with spread sheets and other mathematical software packages.

More advanced reaction kinetics data manipulation may be readily performed with Grafit software. Linear, non-linear or polynomial curve fitting is easily accomplished together with non-linear regression analysis. A wide selection of standard equations are provided, together with an equation editor for customised equation fitting.

Data may also be transformed into other mathematical formats for plotting and calculation.