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Ultra Fast Wavelength Scanning

WaveQuest the CE4300 Dynamic Absorbance Detector (DAD) is a powerful ultra high speed scanning alternative to Photo Diode Array detectors.

Method development, purity determination, R&D and Variable Wavelength Monitoring are possible
with the highest quality.

The design is based on a double beam spectrophotometer concept with ultra fast wavelength scanning over the range 190-700nm. Scans at up to 1000nm/s provide UV spectra in only 200ms.

 
     

Powerful signal processing software enables very high quality ‘on-the-fly’ scans to be triggered by eluting peaks without peak degradation. Eluting peaks may be integrated by PowerStream software at the same time as purity and identification are carried out.

Spectral data is transferred by DataStream software to a PC workstation for spectral manipulation and processing. The software offers library facilities and the export of data. All data may be stored on disk.

Universal UV/Visible Detector

WaveQuest the ultra fast scanning Dynamic Absorbance Detector (DAD) is a major advance in UV-visible detector design, offering the capabilities of a Photo Diode Array (PDA) detector with greatly improved performance when used for chromatogram monitoring.

Very high speed scanning of eluting peaks, “on the fly”, makes purity checks, the determination of optimum monitoring wavelengths for unfamiliar peaks (lambda max scans), and compound identification of peaks using library spectra all possible with considerable cost saving.

One Detector Covers All UV Applications

A single beam Photodiode Array Detector (PDA), used for method development and R & D, will often be replaced by a dual beam UV variable wavelength detector for routine monitoring, when the developed method is adopted, because of the poor drift performance of single beam PDA detectors. Their performance can be as much as 20 times worse. The Dynamic Absorbance Detector has a double beam optical system with very high all round performance and the lowest drift available.

The CE4300 may therefore be used for method development, purity determination, etc. and also as a high performance UV detector for variable wavelength monitoring.

WaveQuest, which is less expensive than a PDA detector, therefore has a double cost advantage.

     
     

40 Ultra Fast Scans of Narrow Peaks

The ultra high speed scanning of the CE4300 is shown. A very fast binary separation was carried
out, on a Cecil gradient elution system 4S, of sixteen polyaromatic hydrocarbons, designated by
the American Environmental Protection Agency as priority pollutants. The time for the elution
was less than 4 minutes, using a 5cm column with a 3 micron packing.

Several of the peaks have widths at half height less than 1.5 seconds. The spectra of all peaks were displayed in real time and are shown here overlayed in groups of four.

   
 
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